RNA Preparation and Purification
Biochain Institute Inc Total RNA - Monkey (Rhesus) Normal Tissue: Colon Ascending, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Apexbio Technology LLC HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)), 25rxns
HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)) is designed for quick production of ARCA capped, 5mCTP and UTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. The addition of 5mCTP (Catalog No. B7967) / UTP (Catalog No. B7972) can reduce host cell immune response. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. The kit contains sufficient reagents to carry out 25 reactions, 20 l each time. Up to 25-180 g of RNA can be generated with 1 g of control template per standard reaction.
Biochain Institute Inc Total RNA - Rat Normal Tissue: Embryo (RtRAN: 17-19 days), 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Small Intestine, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Vagina, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Diabetic Disease: Colon, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Sigma Aldrich Fine Chemicals Biosciences SeqPlex RNA Amplification
The SeqPlex RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (ffPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow.
Research Products International Corp Quick-RNA MicroPrep Kit w/Zymo-Spin IC Columns, Capped, 200 Preps
The Quick-RNA Microprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: DNase I included.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Biochain Institute Inc Total RNA - Alzheimer's Disease: Postcentral Gyrus, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Rat Normal Tissue: Stomach, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Alzheimer's Disease: Parietal Lobe, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zymo Research Corporation Quick-DNA/RNA HT
TheQuick-DNA/RNAHTkit is intended for rapid, high-throughput nucleic acid extraction (automated or manual) from any biological or clinical sample (e.g., swabs – nasal/nasopharyngeal, oropharyngeal, etc.; biological liquids – blood, plasma, serum, saliva, sputum, cells in suspension, etc.; tissue – needle biopsies, LCM, etc.) and/or samples stored in most collection matrices and devices (e.g., UTM, VTM, *DNA/RNA Shield™, RNAlater, RNAProtect, etc.). The kit is compatible with robotic-type sample processors (i.e, bead movers) in combination with sensitive downstream molecular amplification assays. High-quality DNA/RNA extracted with theQuick-DNA/RNAHTkit can be used for Next-Gen sequencing, RT/qPCR and more.
Agilent Technologies High Sensitivity RNA Kit, 1000 samples, 200 to 6000 nt sizing range, 2 μL sample volume
The DNF-472-1000 High Sensitivity RNA Kit is designed for advanced analysis of total RNA and IVT mRNA with Agilent Fragment Analyzer systems. It provides precise RNA quality assessment, crucial for applications such as vaccine development, qPCR, and NGS. This kit allows for reliable sizing and quantification of RNA samples, ensuring its usefulness in various research applications.
- High sensitivity analysis optimized for low-concentration RNA samples.
- Accurate sizing from 200 to 6,000 nt with precise quantification.
- Objective quality assessment using RNA Quality Number (RQN).
- Compatible with Agilent Fragment Analyzer systems for efficient workflow.
- Requires only 2 μL of sample for testing.
Zyagen Labs Human spinal cord total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy HUMAN SPINAL CORD tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Biochain Institute Inc Total RNA - Human Tumor Tissue: Esophagus, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA poly